Application

Detect NMDAR1 using this Anti-NMDAR1 Antibody, (all splice variants), clone R1JHL validated for use in WB.

Research CategoryNeuroscience

Western blot: 1:300. It is recommended that the antibody be diluted in TBST (20 mM Tris-HCl, 137 mM NaCl, 1% Tween-20, pH 7.6) containing 5% nonfat milk and 0.05% sodium azide. The diluted antibody can be used at least 5 times without a significant signal decrease. Store at 2-8°C between uses.

Immunoprecipitation: 3 µg per 200 µg of lysate will quantitatively precipitate all the NMDA R1 in 200 µg of rat brain lysate.

Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurotransmitters & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: (all splice variants)

Fusion protein of amino acids 1-564 from rat NR1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Affinity purified immunoglobulin. Lyophilized. Reconstitute with 50 µL of PBS, pH 7.4. Contains no preservative.

Specificity

NMDA receptor subunit 1 (all splice variants). No cross-reactivity observed with other NMDA receptor subunits. Recognizes a 103-116 kDa protein in rat brain hippocampal homogenate by immunoblot.

Storage and Stability

Maintain lyophilized material at -20°C for up to six months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.